Virus:Article Title: A striosomal accumbens pathway drives stereotyped behavior through an aversive Esr1+ hypothalamic-habenula circuit.
Article Snippet: .. We acknowledge the use of the following virus constructs: AAv phSyn1(S)- FleX- tdtomato- t2A- SypeGFP- WPRe was a gift from h. Zeng (Addgene viral prep #51509- AAv1; http://n2t.net/addgene:51509; RRid:Addgene_51509). peNN.AAv.hSyn.Cre.WPRe.hGh was a gift from J. M. Wilson (Addgene viral prep #105553- AAv1; http://n2t.net/addgene:105553; RRid:Addgene_105553). pAAv- ef1a- fdiO mCherry was a gift from K. deisseroth (Addgene viral prep #114471- AAvrg; http://n2t.net/addgene:114471; RRid:Addgene_114471). pAAv- eF1a- Flpo was a gift from K. deisseroth (Addgene viral prep #55637- AAv1; http://n2t.net/addgene:55637; RRid:Addgene_55637). pAAv- hSyn Con/Fon hChR2(h134R)- eYFP was a gift from K. deisseroth (Addgene viral prep #55645- AAv8; http://n2t.net/addgene:55645; RRid:Addgene_55645). pAAv- eF1a- double floxed- hChR2(h134R)- eYFP- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20298- AAv5; http://n2t.net/addgene:20298; RRid:Addgene_20298). pAAv- eF1a- double floxed- hChR2(h134R)- mCherry- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20297- AAv5; http://n2t.net/addgene:20297; RRid:Addgene_20297). pAAv- eF1a- Cre was a gift from K. deisseroth (Addgene viral prep #55636- AAvrg; http://n2t. net/addgene:55636; RRid:Addgene_55636). pAAv- neF- Con/Fon- NphR3.3- eYFP was a gift from K. deisseroth and iNtRSeCt 2.0 Project (Addgene viral prep #137152- AAv8; http://n2t.net/ addgene:137152; RRid:Addgene_137152). v237 was constructed by h. Wildner, institute of Pharmacology & toxicology, University of Zurich, Switzerland. pAAv- hSyn- FleX- telC- P2Adtomato was a gift from S. datta (Addgene plasmid #159102; http://n2t.net/addgene:159102; RRid:Addgene_159102). .. Funding: this research was supported by the Swedish Research Council 2018- 00608 (vetenskapsrådet Mh, to K.M.), the Swedish Research Council 2023- 01919 (vetenskapsrådet Mh, to d.C.), the Knut and Alice Wallenberg Foundation (KAW, grant D ow nloaded from https://w w w .science.org on N ovem ber 25, 2025 Contesse et al., Sci.
Construct:Article Title: A striosomal accumbens pathway drives stereotyped behavior through an aversive Esr1+ hypothalamic-habenula circuit.
Article Snippet: .. We acknowledge the use of the following virus constructs: AAv phSyn1(S)- FleX- tdtomato- t2A- SypeGFP- WPRe was a gift from h. Zeng (Addgene viral prep #51509- AAv1; http://n2t.net/addgene:51509; RRid:Addgene_51509). peNN.AAv.hSyn.Cre.WPRe.hGh was a gift from J. M. Wilson (Addgene viral prep #105553- AAv1; http://n2t.net/addgene:105553; RRid:Addgene_105553). pAAv- ef1a- fdiO mCherry was a gift from K. deisseroth (Addgene viral prep #114471- AAvrg; http://n2t.net/addgene:114471; RRid:Addgene_114471). pAAv- eF1a- Flpo was a gift from K. deisseroth (Addgene viral prep #55637- AAv1; http://n2t.net/addgene:55637; RRid:Addgene_55637). pAAv- hSyn Con/Fon hChR2(h134R)- eYFP was a gift from K. deisseroth (Addgene viral prep #55645- AAv8; http://n2t.net/addgene:55645; RRid:Addgene_55645). pAAv- eF1a- double floxed- hChR2(h134R)- eYFP- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20298- AAv5; http://n2t.net/addgene:20298; RRid:Addgene_20298). pAAv- eF1a- double floxed- hChR2(h134R)- mCherry- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20297- AAv5; http://n2t.net/addgene:20297; RRid:Addgene_20297). pAAv- eF1a- Cre was a gift from K. deisseroth (Addgene viral prep #55636- AAvrg; http://n2t. net/addgene:55636; RRid:Addgene_55636). pAAv- neF- Con/Fon- NphR3.3- eYFP was a gift from K. deisseroth and iNtRSeCt 2.0 Project (Addgene viral prep #137152- AAv8; http://n2t.net/ addgene:137152; RRid:Addgene_137152). v237 was constructed by h. Wildner, institute of Pharmacology & toxicology, University of Zurich, Switzerland. pAAv- hSyn- FleX- telC- P2Adtomato was a gift from S. datta (Addgene plasmid #159102; http://n2t.net/addgene:159102; RRid:Addgene_159102). .. Funding: this research was supported by the Swedish Research Council 2018- 00608 (vetenskapsrådet Mh, to K.M.), the Swedish Research Council 2023- 01919 (vetenskapsrådet Mh, to d.C.), the Knut and Alice Wallenberg Foundation (KAW, grant D ow nloaded from https://w w w .science.org on N ovem ber 25, 2025 Contesse et al., Sci.
Article Title: The selective amyloid-driven failure of cholinergic medial septal neurons in aging mice perturbs REM sleep, cognition and emotion, and broadcasts amyloid to other brain regions
Article Snippet: These plasmids and their sequences, pAAV-hsyn-DIO- App NL-G-F -P2A-EGFP and pAAV-hsyn-DIO-App NL-G-F -P2A-mCherry, have been deposited at Addgene. .. We also used the following pAAV transgene plasmids: pAAV-hSyn-DIO-EGFP was a gift from Bryan Roth (Addgene plasmid 50457), pAAV-EF1a-flex-taCasp3-TEVp was a gift from Nirao Shah (Addgene plasmid 45580), pAAV-DIO-GCaMP6s transgene was constructed by us and described previously (Addgene plasmid 184284), pAAV-DIO-ChR2-EYFP was a gift from Karl Deisseroth (Addgene plasmid 20298). ..
Plasmid Preparation:Article Title: A striosomal accumbens pathway drives stereotyped behavior through an aversive Esr1+ hypothalamic-habenula circuit.
Article Snippet: .. We acknowledge the use of the following virus constructs: AAv phSyn1(S)- FleX- tdtomato- t2A- SypeGFP- WPRe was a gift from h. Zeng (Addgene viral prep #51509- AAv1; http://n2t.net/addgene:51509; RRid:Addgene_51509). peNN.AAv.hSyn.Cre.WPRe.hGh was a gift from J. M. Wilson (Addgene viral prep #105553- AAv1; http://n2t.net/addgene:105553; RRid:Addgene_105553). pAAv- ef1a- fdiO mCherry was a gift from K. deisseroth (Addgene viral prep #114471- AAvrg; http://n2t.net/addgene:114471; RRid:Addgene_114471). pAAv- eF1a- Flpo was a gift from K. deisseroth (Addgene viral prep #55637- AAv1; http://n2t.net/addgene:55637; RRid:Addgene_55637). pAAv- hSyn Con/Fon hChR2(h134R)- eYFP was a gift from K. deisseroth (Addgene viral prep #55645- AAv8; http://n2t.net/addgene:55645; RRid:Addgene_55645). pAAv- eF1a- double floxed- hChR2(h134R)- eYFP- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20298- AAv5; http://n2t.net/addgene:20298; RRid:Addgene_20298). pAAv- eF1a- double floxed- hChR2(h134R)- mCherry- WPRe- hGhpA was a gift from K. deisseroth (Addgene viral prep #20297- AAv5; http://n2t.net/addgene:20297; RRid:Addgene_20297). pAAv- eF1a- Cre was a gift from K. deisseroth (Addgene viral prep #55636- AAvrg; http://n2t. net/addgene:55636; RRid:Addgene_55636). pAAv- neF- Con/Fon- NphR3.3- eYFP was a gift from K. deisseroth and iNtRSeCt 2.0 Project (Addgene viral prep #137152- AAv8; http://n2t.net/ addgene:137152; RRid:Addgene_137152). v237 was constructed by h. Wildner, institute of Pharmacology & toxicology, University of Zurich, Switzerland. pAAv- hSyn- FleX- telC- P2Adtomato was a gift from S. datta (Addgene plasmid #159102; http://n2t.net/addgene:159102; RRid:Addgene_159102). .. Funding: this research was supported by the Swedish Research Council 2018- 00608 (vetenskapsrådet Mh, to K.M.), the Swedish Research Council 2023- 01919 (vetenskapsrådet Mh, to d.C.), the Knut and Alice Wallenberg Foundation (KAW, grant D ow nloaded from https://w w w .science.org on N ovem ber 25, 2025 Contesse et al., Sci.
Article Title: Post Adversity Changes in Nigro-Striatal Dopamine: A Mechanism for Anxiety Induced Exacerbated Innate Repetitive Behaviors
Article Snippet: .. The expression of the ChR2 opsin allowed us to identify DA neurons projecting to the DLS using phototagging. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth (Addgene plasmid # 20298 ; http://n2t.net/addgene:20298 ; RRID:Addgene_20298). .. To target DA projections to the DMS we used the same vector, again with two depths injection for maximal cover (AP: 0.5, ML: +/-2.5; DV: −2.8, −3.0.
Article Title: The selective amyloid-driven failure of cholinergic medial septal neurons in aging mice perturbs REM sleep, cognition and emotion, and broadcasts amyloid to other brain regions
Article Snippet: These plasmids and their sequences, pAAV-hsyn-DIO- App NL-G-F -P2A-EGFP and pAAV-hsyn-DIO-App NL-G-F -P2A-mCherry, have been deposited at Addgene. .. We also used the following pAAV transgene plasmids: pAAV-hSyn-DIO-EGFP was a gift from Bryan Roth (Addgene plasmid 50457), pAAV-EF1a-flex-taCasp3-TEVp was a gift from Nirao Shah (Addgene plasmid 45580), pAAV-DIO-GCaMP6s transgene was constructed by us and described previously (Addgene plasmid 184284), pAAV-DIO-ChR2-EYFP was a gift from Karl Deisseroth (Addgene plasmid 20298). ..
Injection:Article Title: An anatomical hotspot for striatal dopamine-acetylcholine interactions during reward and movement
Article Snippet: .. Then pAAV-EF1a-doublefloxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Adgene, # 20298), 2.5 x 10 13 GCml - diluted 1:2 in PBS was injected into the right SNc and VTA at 3 sites (200 nl/site at a rate of 100nl/min) at the following three coordinates in mm, AP:-3.05, ML: ± 0.6, DV: -4.25; AP:-3.15, ML: ± 1.3, DV: -4.1; AP:-3.5, ML: ± 1.15, DV: -4.1. ..
Control:Article Title: Semaglutide effects on energy balance are mediated by Adcyap1 + neurons in the dorsal vagal complex.
Article Snippet: Subsequently, bilateral injections were performed at a rate of 5 nl/s (75 nl per depth for TRAP2 and Glp1r-flox, and 25 nl per depth for Adcyap1-2A-Cre mice) using pulled glass pipets and a Nanoject III automated injector (#3-000-207, Drummond Scientific). .. All viral vectors were purchased from Addgene and included for chemogenetic experiments: AAV8 or AAV5-hSyn-DIO-hM3D(Gq)-mCherry and AAV8 or AAV5-hSyn-DIO-mCherry74 (control), for Cre-dependent neuronal ablations: AAV5-EF1a-DIO-taCasp3-TEVp64 and AAV5-hSynDIO-mCherry74 (control), for deletion of GLP-1R expression: AAV8-hSyn-Cre or AAV8-hSyn-mCherry (control) [pAAV-hSyn-DIOhM3D(Gq)-mCherry and pAAV-hSyn-DIO-mCherry were a gift from Bryan Roth (Addgene viral preps #44361-AAV8 and #44361- AAV5, #50459-AAV8 and #50459-AAV5), pAAV-EF1a-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene viral prep #45580-AAV5), pENN.AAV.hSyn.Cre.WPRE.hGH was a gift from James M. Wilson (Addgene viral prep # 105553-AAV8) and pAAV-hSyn-mCherry was a gift from Karl Deisseroth (Addgene viral prep #114472-AAV8)]. .. Subsequently, bilateral injections were performed at a rate of 5 nl/s (75 nl per depth for TRAP2 and Glp1r-flox, and 25 nl per depth for Adcyap1-2A-Cre mice) using pulled glass pipets and a Nanoject III automated injector (#3-000-207, Drummond Scientific).
Expressing:Article Title: Semaglutide effects on energy balance are mediated by Adcyap1 + neurons in the dorsal vagal complex.
Article Snippet: Subsequently, bilateral injections were performed at a rate of 5 nl/s (75 nl per depth for TRAP2 and Glp1r-flox, and 25 nl per depth for Adcyap1-2A-Cre mice) using pulled glass pipets and a Nanoject III automated injector (#3-000-207, Drummond Scientific). .. All viral vectors were purchased from Addgene and included for chemogenetic experiments: AAV8 or AAV5-hSyn-DIO-hM3D(Gq)-mCherry and AAV8 or AAV5-hSyn-DIO-mCherry74 (control), for Cre-dependent neuronal ablations: AAV5-EF1a-DIO-taCasp3-TEVp64 and AAV5-hSynDIO-mCherry74 (control), for deletion of GLP-1R expression: AAV8-hSyn-Cre or AAV8-hSyn-mCherry (control) [pAAV-hSyn-DIOhM3D(Gq)-mCherry and pAAV-hSyn-DIO-mCherry were a gift from Bryan Roth (Addgene viral preps #44361-AAV8 and #44361- AAV5, #50459-AAV8 and #50459-AAV5), pAAV-EF1a-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene viral prep #45580-AAV5), pENN.AAV.hSyn.Cre.WPRE.hGH was a gift from James M. Wilson (Addgene viral prep # 105553-AAV8) and pAAV-hSyn-mCherry was a gift from Karl Deisseroth (Addgene viral prep #114472-AAV8)]. .. Subsequently, bilateral injections were performed at a rate of 5 nl/s (75 nl per depth for TRAP2 and Glp1r-flox, and 25 nl per depth for Adcyap1-2A-Cre mice) using pulled glass pipets and a Nanoject III automated injector (#3-000-207, Drummond Scientific).
Article Title: Post Adversity Changes in Nigro-Striatal Dopamine: A Mechanism for Anxiety Induced Exacerbated Innate Repetitive Behaviors
Article Snippet: .. The expression of the ChR2 opsin allowed us to identify DA neurons projecting to the DLS using phototagging. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth (Addgene plasmid # 20298 ; http://n2t.net/addgene:20298 ; RRID:Addgene_20298). .. To target DA projections to the DMS we used the same vector, again with two depths injection for maximal cover (AP: 0.5, ML: +/-2.5; DV: −2.8, −3.0.
Sequencing:Article Title: Functional Roles of Gastrin-Releasing Peptide-Producing Neurons in the Suprachiasmatic Nucleus: Insights into Photic Entrainment and Circadian Regulation
Article Snippet: .. pcDNASADB19L (#32632), pcDNA-SADB19G (#32633), pcDNA-SADB19N (#32630), pcDNA-SADB19P (#32631), pSADdeltaG-GFP-F2 (#32635), pAAV-EF1a-DIO-hM3D(Gq)-mCherry (#50460), pAAV-EF1a-DIO-mCherry (#50462), pAAV-flex-taCasp3-TEVp (#45580), pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298), and pAAV-Ef1a- DIO-GCaMP6s , pAAV-Ef1a-DIO-GFP were obtained from Addgene. pAAV-CAG-FLEX(Frt)-TVA-mCherry and pAAV-CAG-FLEX(Frt)-RG (rabies glycoprotein; RG) were provided by Naoshige Uchida (Harvard University). pAAV-EF1a-NLS-tdTomato was created by replacing the sequence encoding floxed hM3D(Gq)-mCherry in pAAV-EF1a-DIO-hM3D(Gq)-mCherry with that encoding nuclear localization signals (NLS) and tdTomato using the In-Fusion HD Cloning Kit (CloneTech, catalog #639648). .. The backbone vector and tdTomato coding sequences were amplified using CloneAmp HiFi PCR Premix (CloneTech, catalog #639298).
Cloning:Article Title: Functional Roles of Gastrin-Releasing Peptide-Producing Neurons in the Suprachiasmatic Nucleus: Insights into Photic Entrainment and Circadian Regulation
Article Snippet: .. pcDNASADB19L (#32632), pcDNA-SADB19G (#32633), pcDNA-SADB19N (#32630), pcDNA-SADB19P (#32631), pSADdeltaG-GFP-F2 (#32635), pAAV-EF1a-DIO-hM3D(Gq)-mCherry (#50460), pAAV-EF1a-DIO-mCherry (#50462), pAAV-flex-taCasp3-TEVp (#45580), pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298), and pAAV-Ef1a- DIO-GCaMP6s , pAAV-Ef1a-DIO-GFP were obtained from Addgene. pAAV-CAG-FLEX(Frt)-TVA-mCherry and pAAV-CAG-FLEX(Frt)-RG (rabies glycoprotein; RG) were provided by Naoshige Uchida (Harvard University). pAAV-EF1a-NLS-tdTomato was created by replacing the sequence encoding floxed hM3D(Gq)-mCherry in pAAV-EF1a-DIO-hM3D(Gq)-mCherry with that encoding nuclear localization signals (NLS) and tdTomato using the In-Fusion HD Cloning Kit (CloneTech, catalog #639648). .. The backbone vector and tdTomato coding sequences were amplified using CloneAmp HiFi PCR Premix (CloneTech, catalog #639298).
|
Addgene inc is a verified supplier 